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毕业论文网 > 任务书 > 化学化工与生命科学类 > 食品质量与安全 > 正文

氨基酸对银耳胞外多糖合成的影响任务书

 2020-04-16 04:04  

1. 毕业设计(论文)的内容和要求

内容:建立银耳菌全合成培养基液体发酵工艺条件,对培养条件先进性基础优化,再考察不同种类、不同浓度的氨基酸对银耳多糖产量的影响,确定最适氨基酸添加种类和浓度。

考察ugp酶在银耳多糖合成中的作用。

要求:1.掌握一定的生物化学知识,能够独立完成银耳菌液体发酵;2.掌握一定的基础实验操作,例如测总糖、测葡萄糖浓度等;3.能够使用origin数据处理软件。

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2. 参考文献

[1]Zhu, H., Sun, S.J., 2008. Inhibition of bacterial quorum sensing-regulated behaviors by Tremella fuciformis extract. Curr. Microbiol. 57, 418#8211;422. [2]Cho, E.J., Hwang, H.J., Kim, S.W., Oh, J.Y., Baek, Y.M., Choi, J.W., Bae, S.H., Yun, J.W., 2007. Hypoglycemic effects of exopolysaccharides produced by mycelial cultures of two different mushrooms Tremella fuciformis and Phellinus baumii in ob/ob mice. Appl. Microbiol. Biotechnol. 75, 1257#8211;1265. [3]Kim, J.H., Ha, H.C., Lee, M.S., Kang, J.I., Kim, H.S., Lee, S.Y., Pyun, K.H., Shim, I., 2007.Effect of Tremella fuciformis on the neurite outgrowth of PC12h cells and the improvement of memory in rats. Biol. Pharm. Bull. 30, 708#8211;714. [4]Wu, Y., Shan, L., Yang, S., Ma, A., 2008. Identification and antioxidant activity of melanin isolated from Hypoxylon archeri, a companion fungus of Tremella fuciformis. J. Basic Microbiol. 48, 217#8211;221. [5]Bao, H.N., Ochiai, Y., Ohshima, T., 2010. Antioxidative activities of hydrophilic extracts prepared from the fruiting body and spent culture medium of Flammulina velutipes. Bioresour. Technol. 101, 6248#8211;6255. [6]吴大康,张久春,杨晓丽.银耳深层发酵条件的研究[J].食品科学,2002(01):64-69. [7]袁渊,单麟军,吴尧,曾冰冰,马爱民.培养条件对银耳菌丝体及胞外多糖产量的影响[J].食品工业科技,2010,31(02):242-244. [8]王瑞雪,颜军,邬晓勇,何钢,张良蕾,姚尊伟,苟小军.液体深层发酵条件对银耳菌丝体酸性多糖富集的影响[J].食品科学,2009,30(21):175-177. [9]Hu Zhu , Chunxu Cao , Shuaishuai Zhang , Yan Zhang , Weisheng Zou,2011, pH-control modes in a 5-L stirred-tank bioreactor for cell biomass and exopolysaccharide production by Tremella fuciformis spore, Bioresour. Technol.102(19):9175-9178. [10]何钢. 银耳菌液体发酵工艺优化及卵孢菌素鉴定[D].四川农业大学,2012. [11]郭燕娇. 银耳菌液体培养工艺优化及醪液功能醋饮料研制[D].西华大学,2013. [12]郑良.银耳多糖提取条件及提取液黏度特性的研究[D].四川:四川大学, 2003。

[13]邬雨季,邓佳颖,魏正勋,何荣军,孙培龙,张安强.银耳多糖的制备、功能和产品开发的研究进展[J].食药用菌,2018,26(03):161-165. [14]Eimert K., Villand P., Kilian A., and Kleczkowski L.A., 1996, Cloning and characteri-zation of several cDNAs for UDP-glucose pyrophosphorylase from barley (Hordeum vulgare) tissues, Gene, 170(2): 227-232. [15]张奇茹,丁重阳,周哲敏,顾正华,张梁,石贵阳.葡萄糖-1-磷酸对灵芝多糖合成的影响[J].生物加工过程,2017,15(04):9-14. [16]Jiang SS, Lin T Y, Wang WB,et al , Characterization of UDP-glucose dehydrogenase and UDP-glucose pyrophosphorylase mutants of Proteus mirabilis: defectiveness in polymyxin B resistance, swarming, and virulence[J], Antimicrobial agents and chemotherapy, 2010, 54 (5):2000. [17]Heather D. Coleman,Dave D. Ellis,Margarita Gilbert,Shawn D. Mansfield. Up‐regulation of sucrose synthase and UDP‐glucose pyrophosphorylase impacts plant growth and metabolism[J]. Plant Biotechnology Journal,2006,4(1). [18]Xiaohui Duan,Zhenming Chi,Lin Wang,Xianghong Wang. Influence of different sugars on pullulan production and activities of α-phosphoglucose mutase, UDPG-pyrophosphorylase and glucosyltransferase involved in pullulan synthesis in Aureobasidium pullulans Y68[J]. Carbohydrate Polymers,2008,73(4). [19]Herzberg M,Asperborg H,et al.A transcriptional roadmap to wood formation[J] Proceedings of the National Academy of Sciences, 2001, 98 (25):14732. [20]陈小敏,吴海冰,向泉桔,辜运富,张小平.香菇UDP-葡萄糖焦磷酸化酶转录水平及酶活性对不同碳氮源的响应特征[J].四川大学学报(自然科学版),2018,55(01):214-220.

3. 毕业设计(论文)进程安排

2018年11月 确定选题,查阅资料,拟定实验方案 2018年12月 对银耳菌液体发酵培养基基础优化 2019年1月-3月 研究不同种类、不同浓度的氨基酸对银耳多糖产量的影响,确定最适氨基酸的添加浓度 2019年4月 考察UGP酶在银耳多糖合成中的作用 2019年5月 完成毕业论文编写 2019年6月 准备答辩

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