1. 毕业设计（论文）的内容和要求

要求毕业论文围绕重组纤维二糖差向异构酶的纯化和酶学性质进行阐述，结合实验结果，分析该酶催化底物的基本特性。

为工业化利用重组纤维二糖差向异构酶生产D-阿洛酮糖做技术铺垫。

2. 参考文献

(1) Park, C. S., Kim, J. E., Choi, J. G., Oh, D. K. (2011). Characterization of a recombinant cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus and its application in the production of mannose from glucose. Applied microbiology and biotechnology, 92(6), 1187-1196. (2) Ojima, T., Saburi, W., Sato, H., Yamamoto, T., Mori, H., Matsui, H. (2011). Biochemical characterization of a thermophilic cellobiose 2-epimerase from a thermohalophilic bacterium, Rhodothermus marinus JCM9785. Bioscience, biotechnology, and biochemistry, 75(11), 2162-2168. (3) Ito, S., Taguchi, H., Hamada, S., Kawauchi, S., Ito, H., Senoura, T., ... Matsui, H. (2008). Enzymatic properties of cellobiose 2-epimerase from Ruminococcus albus and the synthesis of rare oligosaccharides by the enzyme. Applied microbiology and biotechnology, 79(3), 433-441.

3. 毕业设计（论文）进程安排

2017.10.10-2017.10.29 确定论文题目、填写任务书 2017.10.30-2017.11.6 毕业设计（论文）任务书及进度表 2017.11.7-2017.12.6 查找资料、写开题报告 2017.12.7-2018.2.12 初步实验及验证 2018.3.1-2018.3.30 完善实验、数据处理 2018.4.1-2018.4.15 论文写作 2018.4.16-2018.4.25 完成论文初稿交老师批阅 2018.4.26-2018.5.15 论文修改 2018.5.16-2018.6.1 论文答辩准备