摘 要

Abstract...........................................................................................................................................ii

第一章 绪论...................................................................................................................................1

1.1硒污染的来源.........................................................................................................................1

1.2硒污染的危害.........................................................................................................................1

1.3微生物还原修复.....................................................................................................................2

第二章 材料与方法.......................................................................................................................4

2.1仪器与试剂.............................................................................................................................4

2.1.1仪器....................................................................................................................................4

2.1.2其他实验用具....................................................................................................................4

2.1.3试剂....................................................................................................................................5

2.1.4培养基与试剂制备............................................................................................................5

2.2实验方法.................................................................................................................................6

2.2.1四价硒的测定方法............................................................................................................6

2.2.2粗提物提取及总蛋白含量测定........................................................................................7

2.2.3四价硒还原酶活性定位....................................................................................................9

2.2.4起始浓度对无细胞提取物还原四价硒的影响........................... ...................................10

2.2.5电子供体对无细胞提取物还原四价硒的影响...............................................................10

2.2.6金属离子对无细胞提取物还原四价硒的影响...............................................................11

2.2.7亚硒酸盐还原酶的动力学常数.......................................................................................11

1. 结果与分析......................................................................................................................11

3.1硒的标准曲线........................................................................................................................11

3.2蛋白的标准曲线和含量测定................................................................................................12

3.3确定四价硒还原酶的细胞定位............................................................................................13

3.4起始浓度对无细胞提取物还原四价硒的影响....................................................................14

3.5电子供体对无细胞提取物还原四价硒的影响....................................................................15

3.6金属离子对无细胞提取物还原四价硒的影响....................................................................17

3.7亚硒酸盐还原酶的动力学常数............................................................................................18

1. 讨论..................................................................................................................................19
2. 结论..................................................................................................................................22

参考文献........................................................................................................................................23

致谢................................................................................................................................................25