全文总字数：2307字

1. 1. 毕业设计（论文）的内容、要求、设计方案、规划等

1.前言

综述木糖苷酶的研究现状，了解目前β-d-木糖苷酶在催化转化7-木糖-10-去乙酰基-紫杉醇中应用情况和酶水解产物分离纯化工艺，主要针对7-木糖-10-去乙酰基-紫杉醇在生物活性、酶催化转化意义、β-d-木糖苷酶在工业上的应用、目的产物分离纯化等方面的研究概况展开综述。了解嗜热微生物来源的木糖苷酶的克隆表达的方式方法和如何通过有效手段提高目的产物的表达量。

2. 实验内容

2. 参考文献（不低于12篇）

[1] hegazy m e f, mohamed t a, elshamy a i, et al. microbial biotransformation as a tool for drug development based on natural products from mevalonic acid pathway: a review[j]. journal of advanced research, 2015, 6(1):17-33.

[2] cheng h l, zhao r y, chen t j, et al. cloning and characterization of the glycoside hydrolases that remove xylosyl groups from 7-β-xylosyl-10-deacetyltaxol and its analogues[j]. molecular cellular proteomics, 2013, 12(8):2236-2248.

[3] dou t y, luan h w, ge g b, et al. functional and structural properties of a novel cellulosome-like multienzyme complex: efficient glycoside hydrolysis of water-insoluble 7-xylosyl-10-deacetylpaclitaxel[j]. scientific reports, 2015, 5(1):13768.