1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解酮脂酰辅酶a的作用与脂肪酸积累的机制，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1]．Fillet S, Ronchel C, Callejo C, et al. Engineering Rhodosporidium toruloides for the production of very long-chain monounsaturated fatty acid-rich oils.[J]. Applied Microbiology Biotechnology, 2017(4):1-10. [2]．Barret P, Delourme R, Renard M, et al. A rapeseed FAE1 gene is linked to the E1 locus associated with variation in the content of erucic acid.[J]. Theoretical Applied Genetics, 1998, 96(2):177-186. [3]．Mietkiewska E, Brost J M, Giblin E M, et al. Cloning and functional characterization of the fatty acid elongase 1 (FAE1) gene from high erucic Crambe abyssinica cv. Prophet.[J]. Plant Biotechnology Journal, 2007, 5(5):636#8211;645. [4]．Guo Y, Mietkiewska E, Francis T, et al. Increase in nervonic acid content in transformed yeast and transgenic plants by introduction of a Lunaria annua L. 3-ketoacyl-CoA synthase (KCS) gene[J]. Plant Molecular Biology, 2009, 69(5):565-572. [5]．Li X, van Loo E N, Gruber J, et al. Development of ultra-high erucic acid oil in the industrial oil crop Crambe abyssinica[J]. Plant Biotechnology Journal, 2012, 10(7):862-870. [6]．Hong S P, Seip J, Walterspollak D, et al. Engineering Yarrowia lipolytica to express secretory invertase with strong FBA1IN promoter.[J]. Yeast, 2012, 29(2):59-72.. [7]．Xue Z, Sharpe P L, Hong S P, et al. Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica.[J]. Nature Biotechnology, 2013, 31(8):734-40. [8]．Blazeck J, Hill A, Liu L, et al. Harnessing Yarrowia lipolytica lipogenesis to create a platform for lipid and biofuel production.[J]. Nature Communications, 2014, 5(1):3131-3140. [9]．Tai M, Stephanopoulos G. Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production.[J]. Metabolic Engineering, 2013, 15(1):1-9. [10]．Qiao K, Imam Abidi S H, Liu H, et al. Engineering lipid overproduction in the oleaginous yeast Yarrowia lipolytica.[J]. Metabolic Engineering, 2015, 29:56-65. [11]．Beopoulos A, Mrozova Z, Thevenieau F, et al. Control of Lipid Accumulation in the Yeast Yarrowia lipolytica[J]. Applied Environmental Microbiology, 2008, 74(24):7779-7789. [12]．Zhang S, Ito M, Skerker J M, et al. Metabolic engineering of the oleaginous yeast Rhodosporidium toruloides IFO0880 for lipid overproduction during high-density fermentation[J]. Applied Microbiology Biotechnology, 2016, 100(21):9393-9405. [13]．Xu P, Qiao K, Stephanopoulos G. Engineering oxidative stress defense pathways to build a robust lipid production platform in Yarrowia lipolytica[J]. Biotechnology Bioengineering, 2017, 114(7):1521-1530. Gao S, Tong Y, Li Z, et al. Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica, for heterologous carotene production[J]. Metabolic Engineering, 2017, 41:192-201.

3. 毕业设计（论文）进程安排

2.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备分子实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写