1. 毕业设计（论文）的内容和要求

乳酸菌抗菌肽是乳酸菌代谢过程中合成并分泌到环境中的一类对革兰氏阳性细菌G ，具有抑制作用的杀菌蛋白或多肽。

gassericin T是一种由加氏乳杆菌分泌的广谱抗菌的抗菌肽，本实验根据加氏乳杆菌的营养需要，采用常用的碳源、氮源、酵母粉以及缓冲盐类，通过响应面方法，对培养基各组分进行优化，为之后高产gassericin T的加氏乳杆菌发酵剂的制备提供基础。

2. 参考文献

1. Radoshevich, L. Cossart, P. Listeria monocytogenes: towards a complete picture of its physiology and pathogenesis. Nature reviews. Microbiology (2017). 2. Rodriguez Plaza, J.G. et al. Cell penetrating peptides and cationic antibacterial peptides: two sides of the same coin. The Journal of biological chemistry 289, 14448-14457 (2014). 3. Vukomanovi#263;, M. et al. Nano-engineering the Antimicrobial Spectrum of Lantibiotics: Activity of Nisin against Gram Negative Bacteria. Scientific Reports 7, 4324 (2017). 4. Eijsink, V.G. et al. Production of class II bacteriocins by lactic acid bacteria; an example of biological warfare and communication. Antonie van Leeuwenhoek 81, 639-654 (2002). 5. Iancu, C. et al. Comparison of the Potency of the Lipid II Targeting Antimicrobials Nisin, Lacticin 3147 and Vancomycin Against Gram-Positive Bacteria. Probiotics and antimicrobial proteins 4, 108-115 (2012). 6. Hoffknecht, B.C. et al. Influence of the Multivalency of Ultrashort Arg-Trp-Based Antimicrobial Peptides (AMP) on Their Antibacterial Activity. ChemMedChem 10, 1564-1569 (2015). 7. Perez, R.H., Zendo, T. Sonomoto, K. Novel bacteriocins from lactic acid bacteria (LAB): various structures and applications. Microbial cell factories 13 Suppl 1, S3 (2014). 8. Shanker, E. Federle, M.J. Quorum Sensing Regulation of Competence and Bacteriocins in Streptococcus pneumoniae and mutans. Genes 8 (2017). 9. Papenfort, K. Bassler, B.L. Quorum sensing signal-response systems in Gram-negative bacteria. Nature reviews. Microbiology 14, 576-588 (2016). 10. Kamareddine, L., Wong, A.C.N., Vanhove, A.S., Hang, S. Purdy, A.E. Activation of Vibrio cholerae quorum sensing promotes survival of an arthropod host. (2017). 11. Jacobi, C.A. et al. Quorum sensing in the probiotic bacterium Escherichia coli Nissle 1917 (Mutaflor) - evidence that furanosyl borate diester (AI-2) is influencing the cytokine expression in the DSS colitis mouse model. Gut pathogens 4, 8 (2012). 12. Ruiz-Barba, J.L., Caballero-Guerrero, B., Maldonado-Barragan, A. Jimenez-Diaz, R. Coculture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an autoinducer-regulated bacteriocin producer, in olive fermentations. Food microbiology 27, 413-417 (2010). 13. Vila-Farres, X., Giralt, E. Vila, J. Update of peptides with antibacterial activity. Current medicinal chemistry 19, 6188-6198 (2012). 14. Huang, Y. Tanaka, T. Characterization of two putative prolinases (PepR1 and PepR2) from Lactobacillus plantarum WCFS1: occurrence of two isozymes with structural similarity and different catalytic properties. Biochimica et biophysica acta 1854, 91-100 (2015). 15. Bohmer, N., Dautel, A., Eisele, T. Fischer, L. Recombinant expression, purification and characterisation of the native glutamate racemase from Lactobacillus plantarum NC8. Protein expression and purification 88, 54-60 (2013). 16. Tsapieva, A., Duplik, N. Suvorov, A. Structure of plantaricin locus of Lactobacillus plantarum 8P-A3. Beneficial microbes 2, 255-261 (2011). 17. Garg, N., Manchanda, G. Kumar, A. Bacterial quorum sensing: circuits and applications. Antonie van Leeuwenhoek 105, 289-305 (2014). 18. Lopes, R. et al. Genome analysis reveals insights of the endophytic Bacillus toyonensis BAC3151 as a potentially novel agent for biocontrol of plant pathogens. 33, 185 (2017). 19. Tada, I., Tanizawa, Y., Endo, A., Tohno, M. Arita, M. Revealing the genomic differences between two subgroups in Lactobacillus gasseri. Bioscience of microbiota, food and health 36, 155-159 (2017). 20. Arakawa, K., Kawai, Y., Nishimura, J., Kitazawa, H. Saito, T. Negative effect of divalent metal cations on production of gassericin T, a bacteriocin produced by Lactobacillus gasseri, in milk-based media. International Dairy Journal 19, 612-616 (2009).

3. 毕业设计（论文）进程安排

2017.2-2017.3 查阅相关文献资料，掌握各种实验仪器设备的使用，开题报告的撰写 2017.3-2017.4 考察不同氮源对乳酸菌的影响 2017.4-2017.5 考察不同氮源对乳酸菌的影响 2017.5-2017.6 应用响应面方法对合成培养基各组分进行优化，进行毕业论文撰写。