登录

  • 登录
  • 忘记密码?点击找回

注册

  • 获取手机验证码 60
  • 注册

找回密码

  • 获取手机验证码60
  • 找回
毕业论文网 > 任务书 > 化学化工与生命科学类 > 应用化学 > 正文

炎症标志物PCT和CRP的免疫层析法双重检测任务书

 2020-06-30 09:06  

1. 毕业设计(论文)的内容和要求

论文内容主要包括绪论部分,对双重检测做一个背景介绍,描述一下pct和crp的免疫层析法双重检测的实验原理以及特点。

第二章主要讲述免疫层析法双重检测的制备过程,主要包括材料,试纸条等的制备。

第三章主要是对双重检测试纸条对pct,crp标准抗原以及实际样本的检测等作详细描述。

剩余内容已隐藏,您需要先支付后才能查看该篇文章全部内容!

2. 参考文献

1. Azarpira N, Ramzi M, Aghdaie M, Daraie M (2009) Procalcitonin and C-reactive protein serum levels after hematopoietic stem-cell transplant. Exp Clin Transplant 7:115?18 2. Agnello L, Bellia C, Di Gangi M, Lo Sasso B, Calvaruso L, Bivona G, Scazzone C, Dones P, Ciaccio M (2016) Utility of serum procalcitonin and C-reactive protein in severity assessment of community-acquired pneumonia in children. Clin Biochem 49:47?0 3. Berlina AN, Taranova NA, Zherdev AV, Venqerov YY, Dzantiev BB (2013) Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk. Anal Bioanal Chem 405:4997?000 4. Bouadma L, Luyt CE, Tubach F, Cracco C, Alvarez A, Schwebel C, Schortgen F, Lasocki S, Veber B, Dehoux M, Bernard M, Pasquet B, Regnier B, Brun-Buisson C, Chastre J, Wolff M, PRORATA trial group (2010) Use of procalcitonin to reduce patients?exposure to antibiotics Qi et al. Nanoscale Research Letters (2016) 11:167 Page 7 of 8 in intensive care units (PRORATA trial): a multicentre randomised controlled trial. Lancet 375:463?74 5. Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013 6. Chan WC, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016?018 7. Celik LH, Demirel FG, Uras N, Oquz SS, Erdeve O, Biyikli Z, Dilmen U (2010) What are the cut-off levels for IL-6 and CRP in neonatal sepsis? J Clin Lab Anal 24:407?12 8. Cheng X, Pu X, Jun P, Zhu X, Zhu D, Chen M (2014) Rapid and quantitative detection of C-reactive protein using quantum dots and immunochromatographic test strips. Int J Nanomedicine 9:5619?626 9. Dandona P, Nix D, Wilson MF, Aljada A, Love J, Assicot M, Bohuon C (1994) Procalcitonin increase after endotoxin injection in normal subjects. J Clin Endocrinol Metab 79:1605?608 10. Eckersall PD, Conner JG, Harvie J (1991) An immunoturbidimetric assay for canine C-reactive protein. Vet Res Commun 15:17?4 11. Fu Y, Chen J, Cai B, Zhang J, Li L, Liu C, Kang Y, Wang L (2012) The use of PCT, CRP, IL-6 and SAA in critically ill patients for an early distinction between candidemia and Gram positive/negative bacteremia. J Infect 64:438?40 12. Garnacho-Montero J, Huici-Moreno MJ, Guti閞rez-Pizarraya A, L髉ez I, M醨quez-V醕aro JA, Macher H, Guerrero JM, Puppo-Moreno A (2014) Prognostic and diagnostic value of eosinopenia, C-reactive protein, procalcitonin, and circulating cell-free DNA in critically ill patients admitted with suspicion of sepsis. Crit Care 18:R116 13. Geszke-Moritz M, Moritz M (2013) Quantum dots as versatile probes in medical sciences: synthesis, modification and properties. Mater Sci Eng C Mater Biol Appl 33:1008?021 14. Hubl W, Krassler J, Zingler C, Pertschy A, Hentschel J, Gerhards-Reich C, Mack M, Demant T (2003) Evaluation of a fully automated procalcitonin chemiluminescence immunoassay. Clin Lab 49:319?27 15. Ledue TB, Weiner DL, Sipe JD, Poulin SE, Collins MF, Rifai N (1998) Analytical evaluation of particle-enhanced immunonephelometric assays for C-reactive protein, serum amyloid A and mannose-binding protein in human serum. Ann Clin Biochem 35:745?53 16. Lei Y, Xiao Q, Huang S, Xu WS, Zhang Z, He ZK, Liu Y, Deng FJ (2011) Impact of CdSe/ZnS quantum dots on the development of zebrafish embryos. J Nanopart Res 13:6895?906 17. Llorente E, Prieto B, Cardo L, Avello N, Alvarez FV (2007) Umbilical cord blood serum procalcitonin by Time-Resolved Amplified Cryptate Emission (TRACE) technology: reference values of a potential marker of vertically transmitted neonatal sepsis. Clin Chem Lab Med 45:1531?535 18. Marchesan S, Prato M (2012) Nanomaterials for (Nano)medicine. ACS Med Chem Lett 4:147?49 19. Morgenthaler NG, Struck J, Fischer-Schulz C, Seidel-Mueller E, Beier W, Bergmann A (2002) Detection of procalcitonin (PCT) in healthy controls and patients with local infection by a sensitive ILMA. Clin Lab 48:263?70 20. Murphy CJ (2002) Optical sensing with quantum dots. Anal Chem 74:520A?26A 21. Rascher D, Geerlof A, Kremmer E, Kr鋗er P, Michael S, Hartmann A, Rieger M (2014) Total internal reflection (TIRF)-based quantification of procalcitonin for sepsis diagnosis梐 point-of-care testing application. Biosens Bioelectron 59:251?58 22. Shen H, Wang H, Tang Z, Niu JZ, Lou S, Du Z, Li LS (2009) High quality synthesis of monodisperse zinc-blende CdSe and CdSe/ZnS nanocrystals with a phosphine-free method. Cryst Eng Comm 11:1733?738 23. Schwenger V, Sis J, Breitbart A, Andrassy K (1998) CRP levels in autoimmune disease can be specified by measurement of procalcitonin. Infection 26:274?76 24. Simon L, Gauvin F, Amre DK, Saint-Louis P, Lacroix J (2004) Serum procalcitonin and C-reactive protein levels as markers of bacterial infection: a systematic review and meta-analysis. Clin Infect Dis 39:206?17 25. Swanson C, D扐ndrea A (2013) Lateral flow assay with near-infrared dye for multiplex detection. Clin Chem 59:641?48 26. Volanakis JE (2001) Human C-reactive protein: expression, structure, and function. Mol Immunol 38:189?97 27. Wang W, He Y, Han Q, Chen A, Hu CM (2013) Preparation of monoclonal antibody against CRP and establishment of a quantitative ELISA for hypersensitive CRP. Immunol J 29:697?01 28. Wegner KD, Hildebrandt N (2015) Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors. Chem Soc Rev 44:4792?834 29. Xu RY, Liu HW, Liu JL, Dong JH (2014) Procalcitonin and C-reactive protein in urinary tract infection diagnosis. BMC Urol 14:45 30. Yu H, Xu L, Qi XP (2014) Quantum dots labeled lateral flow strip in detecting procalcitonin in blood. Chinese J Anal Chem 42:1592?597

3. 毕业设计(论文)进程安排

2017.11.21-11.30 与导师会面,布置论文题目及要求 12.15-1.15 查阅资料,完成开题报告和任务书 2018.3.1-3.5 准备实验所需药品和器材 3.6-3.20 PCT ,CRP检测条件优化,制备标曲 3.21-4.5 多重检测条件摸索,采用间接法检测血清,全血实际样本 4.6-4.20 进行干扰实验以及稳定性检测实验 4.21-5.31 数据整理,书写论文,制作PPT 6.1-6.10 准备论文答辩

剩余内容已隐藏,您需要先支付 10元 才能查看该篇文章全部内容!立即支付

企业微信

Copyright © 2010-2022 毕业论文网 站点地图