1. 毕业设计（论文）的内容和要求

1）查阅NST1基因，基因敲除等相关文献资料； 2）制定详细实验方案，了解整个实验思路，熟悉相关实验操作，并完成开题报告； 3）利用同源重组法敲除酵母中NST1基因，并验证敲除菌是否敲除成功，进行稀释点样实验； 4）整理并分析实验数据，最后撰写毕业论文；

2. 参考文献

[1] Causton HC, Ren B, Koh SS, Harbison CT, Kanin E, Jennings EG, Lee TI, True HL, Lander ES, Young RA. Remodeling of yeast genome expression in response to environmental changes[J]. Mol Biol Cell. 2001, 12(2):323-337. [2] Boone C, Bussey H, Andrews BJ. Exploring genetic interactions and networks with yeast[J]. Nat Rev Genet. 2001, 8(6):437-449. [3] Brush GS, Kelly TJ. Phosphorylation of the replication protein A large subunit in the Saccharomyces cerevisiae checkpoint response[J]. Mol Biol Cell. 2000, 11(12):4277-4294. [4] Molin M et al. Ionizing radiation induces a Yap1-dependent peroxide stress response in yeast. Free Radic Biol Med， 2007，43: 136-144. [5] KLAUNIG J E，KAMENDULIS L M. The role of oxidative stress in carcinogenesis [J]. Annual Review of Pharmacology and Toxicology,2004，44: 239－267． [6] JOMOVA K， BAROS S， VALKO M ． Redox active metal-induced oxidative stress in biological systems［J］.Transition Metal Chemistry，2012，37( 2) : 127 －134. [7] ST-PIERRE J， BUCKINGHAM J A， ROEBUCK S J，et al． Topology of superoxide production from different sites in the mitochondrial electron transport chain［J］ ． The Journal of Biological Chemistry， 2002， 277 ( 47) : 44784 － 44790． [8] E. Gross, D.B. Kastner, C.A. Kaiser, D. Fass, Structure of Ero1p, source of disulfide bonds for oxidative protein folding in the cell, Cell 117 (2004) 601#8211;610. [9] B.P. Tu, J.S. Weissman, The FAD-and O2-dependent reaction cycle of Ero1-mediated oxidative protein folding in the endoplasmic reticulum, Mol. Cell 10 (2002) 983#8211;994. [10] VALKO M ， RHODES C J， MONCOL J， et al． Free radicals， metals and antioxidants in oxidative stress-in-duced cancer［J］．Chemico-Biological Interactions， 2006，160( 1) : 1－40 [11] REUTER S， GUPTA S C， CHATURVEDI M M ，et al．Oxidative stress， inflammation， and cancer: how are they linked? ［J］．Free Radical Biology and Medicine，2010，49( 11) : 1603－1616． [12] KLAUNIG J E， WANG Z， PU X， et al．Oxidative stress and oxidative damage in chemical carcinogenesis［J］．Toxicology and Applied Pharmacology，2011， 254( 2) : 86 － 99．

3. 毕业设计（论文）进程安排

2018.1.1-2018.2.28 文献查阅和撰写开题报告 2018.3.1-2018.5.31 实验研究 2018.6.1-2018.6.10 数据整理和论文写作 2018.6.10-2018.6.15 论文修改和完成答辩