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毕业论文网 > 任务书 > 化学化工与生命科学类 > 药学 > 正文

DNA支架用于体外番茄红素多酶合成体系的构建任务书

 2020-07-01 08:07  

1. 毕业设计(论文)的内容和要求

1.文献查阅 掌握文献查阅的一般方法,学会使用计算机在中国期刊网,维普数据库,超星数字图书馆,elsevier、ncbi电子期刊,springer link全文电子期刊,pubmed等检索资源上查阅关于番茄红素、dna支架等方面的相关文献。

2.文献阅读及综述 阅读与课题相关的外文及中文文献,了解国内外的研究动态,撰写文献综述。

3.明确实验任务,拟定实验方案 根据所查阅文献的内容,确定实验内容及方案,拟定科学可行的研究计划。

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2. 参考文献

[1] Sies H, Stahl W. Vitamins E and C, beta-carotene, and other carotenoids as antioxidants.[J] American Journal of Clinical Nutrition. 1996, 62(6) :1315-1321. [2] 王昆,马玲云,吴先富,肖新月. 番茄红素的研究进展 [J]. 中国药事,2015, 3(29): 266-271. [3] 龚平,阎建全. 番茄红素的研究进展 [J]. 食品与发酵工业,2008, 34(5): 143-147. [4] 张希波,刘洪海,张晓丽,等. 番茄红素化学合成的研究进展 [J]. 西北药学杂志,2009,24(1): 78-80. [5] Reyes L H, Gomez J M, Kao K C. Improving carotenoids production in yeast via adaptive laboratory evolution[J]. Metabolic engineering, 2014, 21: 26-33. [6] Shi F, Zhan W, Li Y, et al. Temperature influences β-carotene production in recombinant Saccharomyces cerevisiae expressing carotenogenic genes from Phaffia rhodozyma[J]. World Journal of Microbiology and Biotechnology, 2014, 30(1): 125-133. [7] Hern#225;ndez-Almanza A, Montantilde;ez J, Mart#237;nez G, et al. Lycopene: Progress in microbial production[J]. Trends in Food Science Technology, 2016, 56: 142-148. [8] Xie W, Ye L, Lv X, et al. Sequential control of biosynthetic pathways for balanced utilization of metabolic intermediates in Saccharomyces cerevisiae[J]. Metabolic engineering, 2015, 28: 8-18. [9] Conrado R J, Wu G C, Boock J T, et al. DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency[J]. Nucleic acids research, 2011, 40(4): 1879-1889. [10] Liu Y, Zhu Y, Ma W, et al. Spatial modulation of key pathway enzymes by DNA-guided scaffold system and respiration chain engineering for improved N-acetylglucosamine production by Bacillus subtilis[J]. Metabolic engineering, 2014, 24: 61-69. [11] Rahman Z, Sung B H, Yi J Y, et al. Enhanced production of n-alkanes in Escherichia coli by spatial organization of biosynthetic pathway enzymes[J]. Journal of Biotechnology, 2014, 192: 187-191. [12] Funabashi H, Yanagi S, Suzuki S, et al. Assembly of zinc finger motif-fused enzymes on a dsDNA scaffold for catalyzing consecutive reactions with a proximity effect[J]. Biotechnology letters, 2015, 37(1): 109-114. [13] Fan L H, Zhang Z J, Yu X Y, et al. Self-surface assembly of cellulosomes with two miniscaffoldins on Saccharomyces cerevisiae for cellulosic ethanol production[J]. Proceedings of the National Academy of Sciences, 2012, 109(33): 13260-13265. [14] Lee J H, Jung S C, Bui L M, et al. Improved production of L-threonine in Escherichia coli by use of a DNA scaffold system[J]. Applied and environmental microbiology, 2013, 79(3): 774-782. [15] Siu K H, Chen R P, Sun Q, et al. Synthetic scaffolds for pathway enhancement[J]. Current opinion in biotechnology, 2015, 36: 98-106.

3. 毕业设计(论文)进程安排

2017.12.20-2018.1.9 文献查询和资料阅读 2018.1.9-2018.1.13 研究方案制定和开题报告写作 2018.1.13-2018.2.25 实践内容的准备 2018.2.25-2018.5.20 实践 2018.5.20-2018.5.25 实践内容处理与结果分析 2018.5.10-2018.6.1 毕业论文书写

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