1. 毕业设计（论文）的内容和要求

1、 毕业论文内容 猪胰脂肪酶在水解反应及转酯化反应中往往具有高的稳定性和活性，其本身对多种有机溶剂、高温、表面活性剂、蛋白酶等有良好的抗性，是目前被广泛应用于洗涤剂添加剂、有机合成和非水相催化等领域中的脂肪酶之一，在实际应用中通常通过固定化处理以实现生产工艺的连续化和自动化。

固定化酶载体材料及其表面修饰是当前酶工程的重要研究内容，复合材料因其优异的性能成为酶固定化领域研究的热点。

本课题拟开展以羧基功能化离子液体为连接桥复合壳聚糖和介孔二氧化硅制备固定化复合载体材料的研究，发展一种新型的环境友好型、高催化效率、高重复使用性固定化载体制备方法，拓展固定化载体的种类及结合方法的应用。

2. 参考文献

[1] Tran D N, Jr. Balkus K J. Perspective of recent progress in immobilization of enzymes[J]. ACS Catalysis, 2011, 1(8): 956-968. [2] Dicosimo R, Mcauliffe J, Poulose A J, et al. Industrial use of immobilized enzymes[J]. Chemical Society Reviews, 2013, 42(15): 6437-6474. [3] Cao X, Li Y, Zhang Z, et al. Catalytic activity and stability of glucose oxidase/horseradish peroxidase co-confined in macroporous silica foam[J]. Analyst, 2012, 137(24): 5785-5791. [4] Ru J, Yi H, He H. Improving the catalytic performance of Porcine pancreatic lipase in the presence of [MMIm][MeSO4] with the modification of functional ionic liquids[J]. Process Biochemistry, 2014, 49(4): 668. [5] Hu Y，Yang J, Jia R, et al. Chemical modification with functionalized ionic liquids: a novel method to improve the enzymatic properties of Candida rugosa lipase[J]. Bioprocess Biosystems Engineering, 2014, 37: 1617. [6] Li X J, Zhang C, Li S, et al. Improving catalytic performance of Candida rugosa lipase by chemical modification with polyethylene glycol functional ionic liquids[J]. Industrial Engineering Chemistry Research, 2015, 54: 8072. [7] Hu W, Guan Z, Deng X, et al. Enzyme catalytic promiscuity: The papain-catalyzed Knoevenagel reaction[J]. Biochimie, 2012, 94 (3): 656-661. [8] 丁雁, 黄和, 胡燚. 脂肪酶催化 C#8212;C 键形成反应研究新进展[J]. 有机化学, 2013, 33(5): 905-914. [9] Payentko V, Matkovsky A, Matrunchik Y. Composites of silica with immobilized cholinesterase incorporated into polymeric shell[J]. Nanoscale Research Letters, 2015, 10(82): 1-3. [10] Singh V, Singh D. Polyvinyl alcohol-silica nanohybrids: An efficient carrier matrix for amylase immobilization[J]. Process Biochemistry, 2013, 48(1): 96-102. [11] Xie W, Zang X. Immobilized lipase on core-shell structured Fe3O4-MCM-41 nanocomposites as a magnetically recyclable biocatalyst for interesterification of soybean oil and lard[J]. Food Chemistry. 2016, 194: 1283-1292. [12] Yang L, Guo Y, Zhan W, Guo Y, Wang Y, Lu G. One-pot synthesis of aldehyde-functionalized mesoporous silica-Fe3O4 nanocomposites for immobilization of penicillin G acylase[J]. Microporous and Mesoporous Materials. 2014, 197: 1-7.

3. 毕业设计（论文）进程安排

2017.1-2017.1 完成文献检索和开题报告 2017.2-2017.3 建立离子液体制备及表面修饰方法和对猪胰脂肪酶进行纯化 2017.3-2017.4 测定筛选出的脂肪酶酶学性能（热稳定性、储藏稳定性、pH耐受性、催化效率、动力学参数），制备羧基功能化介孔载体及与壳聚糖复合，制备固定化猪胰脂肪酶 2017.4-2017.5 测定固定化猪胰脂肪酶性能并与游离酶性能进行对比，对材料复合前后及固定化酶前后的材料表征 2017.6 数据整理、论文写作和答辩