1. 毕业设计（论文）的内容和要求

论文内容

阿斯巴甜(aspartame，apm)，作为蔗糖的重要替代品，甜度约为蔗糖的200倍，但其热值仅为蔗糖的1/200。已经成为国际市场上的主导强力甜味剂，前期筛选到的来源于pseudomonas aeruginosa 所产蛋白酶pt121具有高效催化阿斯巴甜前体(z-apm)的潜在能力，本课题拟通过定点突变提高催化z-阿斯巴甜合成。

论文要求

2. 参考文献

[1] liu lw , wang b , chen hg, wang sy, wang md , zhang sm,song ad, shen jw, wu k, jia xc. rationalph - engineering of the thermostable xylanase bas ed on computational model. process biochemistry 44 (2009) 912#8211;915.

[2] zhan dl, han ww, feng y. experimental and computational studies indicate the mutation of glu12 to increase the thermostability of oligomeric protease from pyrococcushorikoshii. j mol model, 2011; 17(6): 1241-9.

[3] tian j, wang p, gao s, chu x, wu n, fan y. enhanced thermostability of methyl parathion hydrolase from ochrobactrum sp. m231 by rational engineering of a glycine to proline mutation. febs journal 2010; 277(23), 4901#8211;8.

3. 毕业设计（论文）进程安排