1. 毕业设计（论文）的内容和要求

1、 内容 在试验过程中，制定出一套试验方案，也就是具体步骤，即：1、精子细胞的收集与处理2、气相色谱-质谱联用仪（gc-ms）进样分析3、数据处理与分析。

本实验以人精子细胞为研究对象，研究精子细胞内小分子代谢物群，探讨活力低下的精子与正常精子间的差异，并寻找潜在的生物标志物。

2、 要求 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier电子期刊，pub med，sci等检索资源上查阅关于细胞代谢组学的相关文献。

2. 参考文献

1. J C L J. K. Nicholson, E Holmes. 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J]. Xenobiotica, 1999,29(11). 2. D J Crockford, E Holmes, J C Lindon, et al. Statistical Heterospectroscopy, an Approach to the Integrated Analysis of NMR and UPLC-MS Data Sets: Application in Metabonomic Toxicology Studies[J]. Analytical Chemistry, 2006,78(2):363~371. 3. Williams R, Lenz EM, Wilson AJ, et al. A multi-analytical platform approach to the metabonomic analysis of plasma from normal and zucker (fa/fa) obese rats[J]. Mol Biosyst, 2006,2(3-4):174~183. 4. L Eriksson, H Antti, J Gottfries, et al. Using chemometrics for navigating in the large data sets of genomics, proteomics, and metabonomics (gpm)[J]. Analytical and Bioanalytical Chemistry, 2004,380(3):419~429. 5. R Madsen, T Lundstedt, J Trygg. Chemometrics in metabolomics#8212;A review in human disease diagnosis[J]. Analytica Chimica Acta, 2010,659(1-2):23~33. 6. Armitage EG and Barbas C. Metabolomics in cancer biomarker discovery: Current trends and future perspectives. J Pharmaceut Biomed. 2014; 87:1-11. 7. Cox DG, Oh J, Keasling A, Colson KL and Hamann MT. The utility of metabolomics in natural product and biomarker characterization. Bba-Gen Subjects. 2014; 1840(12):3460-3474. 8. Metz TO, Zhang QB, Page JS, Shen YF, Callister SJ, Jacobs JM and Smith RD. Future of liquid chromatography-mass spectrometry in metabolic profiling and metabolomic studies for biomarker discovery. Biomark Med. 2007; 1(1):159-185. 9. Dieterle F, Riefke B, Schlotterbeck G, Ross A, Senn H and Amberg A. NMR and MS methods for metabonomics. Methods in molecular biology. 2011; 691:385-415. 10. Zhang AH, Sun H, Wang P, Han Y and Wang XJ. Modern analytical techniques in metabolomics analysis. Analyst. 2012; 137(2):293-300. 11. Sreekumar A, Poisson LM, Rajendiran TM, Khan AP, Cao Q, Yu JD, Laxman B, Mehra R, Lonigro RJ, Li Y, Nyati MK, Ahsan A, Kalyana-Sundaram S, Han B, Cao XH, Byun J, et al. Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression. Nature. 2009; 457(7231):910-914. 12. Brockmoller SF, Bucher E, Muller BM, Budczies J, Hilvo M, Griffin JL, Oresic M, Kallioniemi O, Iljin K, Loibl S, Darb-Esfahani S, Sinn BV, Klauschen F, Prinzler J, Bangemann N, Ismaeel F, et al. Integration of Metabolomics and Expression of Glycerol-3-phosphate Acyltransferase (GPAM) in Breast Cancer-Link to Patient Survival, Hormone Receptor Status, and Metabolic Profiling. J Proteome Res. 2012; 11(2):850-860. 13. Gilany K, Moazeni-Pourasil RS, Jafarzadeh N and Savadi-Shiraz E. Metabolomics Fingerprinting of the Human Seminal Plasma of Asthenozoospermic Patients. Mol Reprod Dev. 2014; 81(1):84-86. 14. Jafarzadeh N, Mani-Varnosfaderani A, Minai-Tehrani A, Savadi-Shiraz E, Sadeghi MR and Gilany K. Metabolomics Fingerprinting of Seminal Plasma from Unexplained Infertile Men: A Need for Novel Diagnostic Biomarkers. Mol Reprod Dev. 2015; 82(3):150-150. 15. Zhang XL, Diao RY, Zhu XY, Li ZS and Cai ZM. Metabolic characterization of asthenozoospermia using nontargeted seminal plasma metabolomics. Clin Chim Acta. 2015; 450:254-261. 16. Am-In N, Kirkwood RN, Techakumphu M and Tantasuparuk W. Lipid profiles of sperm and seminal plasma from boars having normal or low sperm motility. Theriogenology. 2011; 75(5):897-903. 17. Lu JC, Jing J, Yao Q, Fan K, Wang GH, Feng RX, Liang YJ, Chen L, Ge YF and Yao B. Relationship between Lipids Levels of Serum and Seminal Plasma and Semen Parameters in 631 Chinese Subfertile Men. Plos One. 2016; 11(1). 18. Kim N, Nakamura H, Masaki H, Kumasawa K, Hirano K and Kimura T. Effect of lipid metabolism on male fertility. Biochem Bioph Res Co. 2017; 485(3):686-692.

3. 毕业设计（论文）进程安排

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