1. 毕业设计（论文）的内容和要求

1、 内容 在试验过程中，制定出一套试验方案，也就是具体步骤，即：1、细胞培养（换液与传代）2、建立裸鼠异种移植瘤模型3、动物给药干预4、动物实验相关指标测定5、数据处理。

本研究以人骨肉瘤细胞为研究对象，建立裸鼠异种移植瘤模型，以冬凌草甲素进行治疗，对裸鼠的各项指标进行测定，并对测得数据进行处理。

2、要求 （1）掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，sci等检索资源上查阅关于细胞凋亡的相关文献。

2. 参考文献

1. Wittig JC, Bickels J, Priebat D, Jelinek J, Kellar#8209;Graney K, Shmookler B, Malawer MM. Osteosarcoma: A multidisciplinary approach to diagnosis and treatment. Am Fam Physician 2002; 65:1123#8209;32. 2. de la Taille A, Hayek OR, Burchardt M, Burchardt T, Katz AE. Role of herbal compounds (PC#8209;SPES) in hormone#8209;refractory prostate cancer: Two case reports. J Altern Complement Med 2000; 6:449#8209;51. 3. Li XT, Lin C, Li PY. Characteristics of the cytostatic effects of oridonin in vitro. Acta Pharmacol Sin 1986; 7:361#8209;3. 4. Ikezoe T, Chen S, Heber D, Taguchi H, Koeffler HP. Baicalin is a major component of PC#8209;SPES which inhibits the proliferation of human cancer cells via apoptosis and cell cycle arrest. Prostate 2001; 49:285#8209;92. 5. Hsieh TC, Wijeratne EK, Liang JY, Gunatilaka AL, Wu JM. Differential control of growth, cell cycle progression, and expression of NF#8209;kappaB in human breast cancer cells MCF#8209;7, MCF#8209;10A, and MDA#8209;MB#8209;231 by ponicidin and oridonin, diterpenoids from the chinese herb Rabdosia rubescens. Biochem Biophys Res Commun 2005; 337:224#8209;31. 6. Zhang JF, Liu JJ, Liu PQ, Lin DJ, Li XD, Chen GH. Oridonin inhibits cell growth by induction of apoptosis on human hepatocellular carcinoma BEL#8209;7402 cells. Hepatol Res 2006; 35:104#8209;10. 7. Leung CH, Grill SP, Lam W, Han QB, Sun HD, Cheng YC. Novel mechanism of inhibition of nuclear factor#8209;kappa B DNA#8209;binding activity by diterpenoids isolated from Isodon rubescens. Mol Pharmacol 2005; 68:286#8209;97. 8. Ikezoe T, Yang Y, Bandobashi K, Saito T, Takemoto S, Machida H, Togitani K, Koeffler HP, Taguchi H. Oridonin, a diterpenoid purified from Rabdosia rubescens, inhibits the proliferation of cells from lymphoid malignancies in association with blockade of the NF#8209;kappa B signal pathways. Mol Cancer Ther 2005; 4:578#8209;86. 9. Liu YQ, You S, Tashiro S, Onodera S, Ikejima T. Activation of phosphoinositide 3#8209;kinase, protein kinase C, and extracellular signal#8209;regulated kinase is required for oridonin#8209;enhanced phagocytosis of apoptotic bodies in human macrophage#8209;like U937 cells. J Pharmacol Sci 2005; 98:361#8209;71. 10. Vivanco I, Sawyers CL. The phosphatidylinositol 3#8209;kinase AKT pathway in human cancer. Nat Rev Cancer 2002; 2:489#8209;501.

3. 毕业设计（论文）进程安排

2月 确定毕业设计的课题及相关资料的查找 3月 完成相关的材料准备；完成开题报告 实验方法的确定及实验条件的优化 4月 实验阶段 5月 各项指标作比较，实验数据处理 6月 毕业论文的书写 在导师指导下修改论文，完成答辩